Kuyesa Kwatsopano Kwatsopano kwa Handheld Covid-19 Nucleic Acid

Posachedwapa, kampani ya Pluslife Biotech, yomwe ili ku Greater Bay Area, idakhazikitsa mayeso oyamba am'manja a Covid-19 Nucleic Acid Test kuti athandizire kupewa ndi kuwongolera miliri.         

Hong Kong yakhudzidwa kwambiri ndi funde laposachedwa la matenda a COVID-19 m'masabata aposachedwa. Pothana ndi zovuta kuphatikiza kusowa kwa anthu ogwira ntchito popewera ndi kuwongolera miliri, komanso kuchepa kwa kuyesa, boma la Hong Kong SAR lakhazikitsa pulogalamu yapadziko lonse lapansi, kugawa zida zoyesera ndikulola okhalamo kuti adziyese okha ku COVID-19. Amene akufunika kutsimikizira zotsatira atha kupita kumalo oyesera kuti akapeze mayeso owonjezera a qPCR.

The Diagnosis and Treatment Protocol for COVID-19 (Trial Version 8), yotulutsidwa pamodzi ndi National Health Commission and National Administration of Traditional Chinese Medicine, ikunena momveka bwino kuti zotsatira zabwino za kuyesa kwa nucleic acid ndiye njira yayikulu yodziwira COVID-19. Poyerekeza ndi kuyesa kwa antigen mwachangu, kukhudzika ndi kutsimikizika kwa mayeso a nucleic acid ndizopambana kwambiri, ndipo zimatha kuzindikira odwala omwe ali ndi kachilombo koyambirira. Komabe, kuyesa kwaposachedwa kwa nucleic acid ngati kuyesa kwa qPCR kumafuna zida zodula komanso njira zovutirapo, chifukwa chake mayesowa amagwiritsidwa ntchito m'zipatala, m'malo oyesera anthu ena kapena ma laboratories. Chifukwa chake, kuyesa kwa qPCR sikuthandiza kupeza zotsatira zoyeserera nthawi yomweyo pamalo oyeserera a anthu.

Ngakhale kuyesa kwa antigen mwachangu ndikosavuta komanso kosavuta kugwiritsa ntchito, kukhudzika kwake ndikotsika kwambiri kuposa kuyesa kwa qPCR. Nthawi zambiri, imatha kuzindikira zitsanzo zabwino zokha, ndipo ngati kuchuluka kwa ma virus omwe ali muzoyeserera sikufika pa nambala inayake, padzakhala mwayi wabodza. Chifukwa chake, koyambirira kwa matenda, kuyesa kwa qPCR ndikolondola kwambiri kuposa kuyesa kwa antigen.

Pulofesa Zhou SONGYANG, Woyambitsa Pluslife Biotech, adati, "Ndi kufalikira kwachangu kwa COVID-19 komanso kufunikira kowunika kwa COVID-19, kupanga zida zoyenera za nucleic acid Point of Care Testing (POCT) zomwe ndizotsika mtengo komanso zosavuta monga. Kuyesa kwa antigen mwachangu, ndikusunga kulondola komanso kukhudzika komwe kumayesedwa kwa qPCR, kungakhale kothandiza kwa anthu onse kuti athe kuthana ndi mliriwu. ”

Pluslife Biotech ndi wopanga komanso wopanga zoyesa za POCT nucleic acid & zoyesa kunyumba zochokera ku Greater Bay Area. Kampaniyo yatumiza mwachangu gulu loyamba la zida zoyesera masauzande kuti zigwiritsidwe ntchito kuzipatala ndi zipatala za Hong Kong. Pluslife Biotech ndi mphamvu yatsopano yolimbana ndi mliriwu pogwiritsa ntchito ukadaulo, ndipo imodzi mwamakampani oyamba ku China kuyambitsa kuyesa kwa in vitro diagnostic (IVD) kunyumba kwa POCT nucleic acid. Pluslife Mini Dock, yopangidwa ndi Pluslife Biotech, ndiye chida choyamba choyesera cha POCT nucleic acid cha COVID-19. Kampaniyo yamaliza kasamalidwe kabwino ka ISO13485 ndi satifiketi ya CE ndikugulitsa m'magawo ambiri padziko lonse lapansi.

Kuyesa kwa Pluslife kwa POCT nucleic acid kumafika pamlingo wokhudzika kwambiri, kutsimikizika komanso kulondola, komwe kumafanana ndi kuyesa kwa qPCR. Imathanso kuzindikira mokhazikika ma virus pa LoD yotsika kwambiri (Limit of Detection). LoD yokhazikika yokhazikika ndi makope 200/mL, yomwe ili yabwinoko kuposa kuyesa kwa qPCR.

Kuphatikiza apo, Pluslife Mini Dock imayang'anira zovuta zomwe zilipo zodalira zida zodula (gawo limodzi nthawi zambiri limakhala lamtengo wopitilira mazana masauzande a madola aku Hong Kong), ndipo limatha kuyesa kuyesa kwa nucleic acid pamalo oyambira ndikupeza zotsatira zoyesa. nthawi yomweyo. Pankhani ya njira yoyesera, mutatha kutenga chitsanzo cha m'mphuno cham'mbuyo, ogwiritsa ntchito amangoyika swab mu lysate ndi khadi loyesa, ndiyeno amaika khadi loyesa mu Mini Dock kuti ayese kuyesa kamodzi ndikupeza zotsatira.

Pakuyesa kuyezetsa bwino, Pluslife Mini Dock imatha kuzindikira zitsanzo zabwino mkati mwa mphindi 15 ndikutsimikizira chitsanzo cholakwika mumphindi 35, kuchepetsa kwambiri nthawi yodikirira poyerekeza ndi mayeso a qPCR (nthawi zambiri maola 3-4 ndikuchotsa, kuphatikiza kusamutsa zitsanzo. nthawi yopita ku labotale). Pankhani ya mtengo, mtengo wa Pluslife Mini Dock ndiokwera mtengo kwambiri kuposa zida zina zoyesera za POCT nucleic acid pamsika, komanso zimatha kugwiritsidwanso ntchito, ndikupangitsa kuti ikhale yoyenera kugwiritsidwa ntchito mokulira pamunsi.

Kupita patsogolo kwa zinthu za Pluslife Biotech zomwe zimakhudzidwa kwambiri, zotsika mtengo, zosavuta kugwiritsa ntchito komanso zodalirika zimathandizidwa ndi gulu lomwe lili ndi luso lamphamvu komanso lodzipereka kwambiri paukadaulo.

Pulofesa Zhou SONGYANG ali ndi zaka zambiri zaukatswiri pa kafukufuku ndi chitukuko pankhani ya uinjiniya wa mapuloteni ndi madera ena a sayansi ya moyo. Wasindikiza zolemba zoposa 150 monga wolemba woyamba komanso wolemba wofanana nawo m'magazini odziwika bwino padziko lonse lapansi monga Cell, Nature and Science, ndi mawu opitilira 19,000. Gulu la R&D la kampaniyo limaphatikizapo gulu loyamba la matalente apamwamba akumayiko akunja, maprofesa, ma PhD ndi akatswiri akulu pantchito ya IVD, omwe ali ndi chidziwitso chochulukirapo pamapuloteni oyambira, matekinoloje oyesa, kapangidwe kazinthu komanso kupanga kokhazikika.

Zogulitsa zachikhalidwe za qPCR zimadalira kutentha kwapamwamba ndipo zimakhala ndi zofunikira za hardware, zomwe zimapangitsa kuti zidazo zikhale zokwera mtengo; pomwe kuyesa komwe kulipo kwa isothermal nucleic acid kumatha kuthana ndi vuto la mtengowo ndikuthamanga mwachangu, koma sikungafikire kukhudzika kwabwino komanso kutsimikizika, zomwe zimawapangitsa kulephera kuzindikirika mwachindunji ndi qPCR, kotero kwa nthawi yayitali kunalibe. kuyezetsa kopangidwa bwino kwa POCT nucleic acid komwe kungagwiritsidwe ntchito m'mabanja komanso zipatala zamagulu ammudzi.

Kuti apange chinthu choyezera kwambiri cha POCT nucleic acid, Pluslife Biotech idapanga RHAM, ukadaulo woyambira wokhala ndi ufulu wodziyimira pawokha waluso, womwe ndi wosiyana ndi umisiri wanthawi zonse wokulitsa mphamvu ya isothermal monga ukadaulo wa LAMP kapena CRISPR.

Ukadaulo wa RHAM ukuwonetsa magwiridwe antchito ofanana ndi a qPCR, ndipo ndiabwino kwambiri kuposa matekinoloje achikhalidwe a isothermal amplification (monga LAMP) potengera kukhudzika, kukhazikika komanso kutsimikizika. Kulekerera kwakukulu komanso kuyanjana kwabwino kwa RHAM kumazindikira magwiridwe antchito a gawo limodzi la kukonza zitsanzo, kukulitsa ndi kuzindikira zonse m'modzi. Izi sizimaphatikizapo zochita monga kutsegula chivindikiro pambuyo pa kukulitsa (palibe kuipitsidwa kwa aerosol), ndipo ili ndi zofunikira zochepa za chilengedwe chakunja ndi chithandizo cha hardware. Pakadali pano, Pluslife Biotech yafunsira ma patent opitilira 60 pamatekinoloje osiyanasiyana kuphatikiza RHAM, omwe ambiri aperekedwa.

Zoyeserera za COVID-19, zoimiridwa ndi Pluslife Mini Dock, zatsegula mwayi woyeserera wa POCT nucleic acid. Malinga ndi Pulofesa Zhou SONGYANG, Pluslife Mini Dock ingagwiritsidwe ntchito pazochitika monga miyambo, malo oyezera ndege, zochitika zadzidzidzi zachipatala, kuyezetsa kachipatala mwamsanga, ma lab mobile / field / kuyezetsa ndi asilikali, zipatala zamagulu, komanso ngakhale kudziyesa kunyumba. Kupyolera mu kuyezetsa kosinthika kwapamalo, kupewa ndi kuwongolera miliri kumatha kupezedwa poyambira. Odwala a COVID-19 amathanso kuzindikirika ndikuikidwa kwaokha msanga, ndikuchepetsa nthawi yodikirira omwe ali ndi zotsatira zoyipa.